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The membranes were washed with TBST and incubated for 2 ~ 2.5 h at 25 C with an anti-rabbit (mouse) immunoglobulin G horseradish peroxidase-conjugated secondary antibody (1:5000 dilution

The late development and maturation are related to the late cortical gliogenesis and convert brain cortical function from being purely neuronal to becoming neuronal-astrocytic

10.1016/j.biopha.2018.09.095 90
