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The human CTR was modified to contain an N-terminal Flag tag epitope and a C-terminal 8 histidine tag epitope, each flanked by a HRV 3 C protease cleavage sites

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However, we also observe an increase in the flow of TCA cycle metabolites, via the non-oxidative branch of the TCA cycle, into ribose labeling

Taken together, this study highlights the potential complementary roles of prescribed SOS and methadone as options in response to the urgent drug toxicity crisis, particularly given the higher medical complexity and high rates of recent and concurrent OAT among people receiving SOS