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Based on previous reports and preliminary experiments conducted in our lab, the treatments tested were IGF-I at 100 ngmL 1 (Wasielak et al., 1 ) (Yeste et al., In experiment 1, cumulusoocyte complexes (COCs) obtained from pre-pubertal gilts ovary were selected and matured in vitro in conventional IVM medium (control) or in the same medium supplemented with 100 ngmL 1 IGF-I

HCG plays a crucial role in maintaining fertility, testicular function, and natural hormone production

Ensuring the bottle remains tightly sealed after each use helps preserve freshness and maintain the formula's intended consistency

Bacteriostatic water preserves stability for 28 days under refrigeration