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A Vitrobot Mark IV (FEI) operating at 4C, 100% humidity, wait time = 10 seconds, blot force = 4, blot time = 4 sec was used for blotting with grade 595 filter paper (Agar Scientific cat#AG47000) before plunging into liquid ethane

To confirm whether hypoxia induces Rv0884c and serine production during in vivo infection, the oxygen tension in the lung tissues and dLNs of C57BL/6 mice was assessed using pimonidazole (PIMO) staining
And make sure to protect it
