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The report was uncontrolled, unblinded, not randomized, had no comparator arm, used patient-reported outcomes rather than validated pain instruments, and did not publish dose, effect size, confidence intervals, p-values, or systematically collected adverse events limitations that severely constrain any clinical inference

Lopes S, Jurisicova A, Sun JG, Casper RF: Reactive oxygen species: potential cause for DNA fragmentation in human spermatozoa

Precise for low starting doses (0.5mg2mg)

Semi-automated freeze-substitution was performed using an AFS2 device (Leica) equipped with customized aluminum sapphire disc revolvers and samples were subsequently embedded in epoxy resin according to a previously published protocol 73