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The heterogeneous manifestations of HE vary not only between patients but also longitudinally for an individual patient

In brief, mice were euthanized by CO 2 inhalation, and the soleus and gastrocnemius muscles were dissected and immediately washed in 2 mL of ice-cold IB3 buffer (225 mM mannitol, 75 mM sucrose, 30 mM Tris-HCl, pH 7.4), then stored in fresh ice-cold IB3 buffer

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