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Following complete dissolution, 300 mL of isobutanol were added and the resulting mixture concentrated under vacuum at 40 C
TMAO also induces endothelial dysfunction by disrupting junction proteins, activating the NLRP3 inflammasome to release high-mobility group box 1 protein (HMGB1), and altering endothelial permeability (Singh et al., 2019)
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The receiver operator characteristic curve analysis (AUROC) demonstrated good discriminating power of the classification model to distinguish the +ADT from the ADT group [Figure 1D: AUC = 0.839, 95% CI (0.725, 0.903)]
