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Primary human fibroblasts (Supplementary Table 6) were grown in DMEM supplemented with 20% FBS, 0.1 mM nonessential amino acids (Invitrogen), 2 mM L-glutamine (Invitrogen) and penicillin/streptomycin

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Low glutathione levels in cystic fibrosis and in smokers contribute to local inflammation and detrimental effects in the lung.[i] Glutathione, oxidative stress, and COVID-19[ii] Infection with SARS-CoV-2 causes COVID-19 (coronavirus disease 2019) 14% of individuals develop severe disease 5% develop critical disease Majority are asymptomatic or have mild to moderate symptoms More severe forms are seen in those who are older or have comorbidities Increased oxidative stress, impaired redox homeostasis, inflammation, antioxidant and glutathione insufficiency contribute to chronic comorbidities (e.g