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pcDNA-Cyto-TerminaTOR, pcDNA-Lyso-TerminaTOR and pcDNA-Nuc-TerminaTOR were subcloned into a doxycycline-inducible lentiviral-expressing vector (Addgene, backbone 27565) using Gibson assembly, yielding pLenti-TetOn-Cyto-TerminaTOR, pLenti-TetOn-Lyso-TerminaTOR and pLenti-TetOn-Nuc-TerminaTOR

Alcohol consumption contributes to dehydration, poor sleep, nutrient depletion, and inflammation

Critically, our work shows that compounds must be carefully selected based on their MOA and evaluated for inclusion within a chemotherapeutic protocol

In addition, mouse mast cell protease (MMCP)-6 and MMCP-7 triggered the release of IL-33 from glia-neuron mixed cultures and primary mouse astrocytes