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Aspirin-induced alterations in GSH metabolism GSH-Px activity in HepG2 cells was significantly increased after ASA treatment (Figure 5A and B).The enzyme activity was further increased (40%) in GSH-depleted- 5 mol/ml ASA treated cells and almost 2-fold in GSH-depleted 10 mol/ml ASA treated cells in the PMS while mitochondria showed only 1530% increased activity in GSH-depleted cells