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40 juvenile male Western Albino rats (aged 34Ramaekers et al., weeks) received oral PPA at 250 mg/kg/for 3 days to induce autism-like features, followed by 3 weeks of probiotic treatment with Bifidobacterium infantis , Lactobacillus bulgaricus, or a commercial probiotic mixture (all at 0.2 g/kg)

Although folic acid is better absorbed across the digestive tract, both forms enter the same biochemical pathway and are indistinguishable at the cellular level