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Human trials have predominantly used topical or brief systemic dosing, and tolerability is overall reported to be good

Abdel-Daim MM, Khalifa HA, Abushouk AI et al (2017) Diosmin attenuates methotrexate-nduced hepatic, renal, and cardiac njury: a biochemical and histopathological study in mice

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The next day, cells were washed and incubated for 90 min at room temperature with Alexa Fluor-conjugated secondary antibodies (anti-mouse Alexa Fluor 555, Invitrogen