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The plate was immediately incubated at 37 C in a humidified atmosphere with 5% CO 2 for 4 h

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Common symptoms include: Persistent fatigue Low energy levels Shortness of breath Dizziness or feeling faint Irregular heartbeats (palpitations) Pale or yellowish skin Reduced appetite or unintentional weight loss If left untreated, B12 deficiency can lead to serious complications, including nerve damage, cognitive issues, and changes in mood