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Purification of FLVCR2 for cryo-EM Frozen membranes were thawed on ice and solubilized in basis buffer (200 mM NaCl, 50 mM TrisHCl, pH 7.5, 10% glycerol, 5 mM choline chloride, 20 mM imidazole) containing 0.7% DDM and 0.07% CHS for 1 h at 4C

J Cardiovasc Pharmacol 1992;20:157-64

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[DOI] [PubMed] [Google Scholar] 51.Moog-Lutz C, Tomasetto C, Regnier CH, Wendling C, Lutz Y, Muller D, Chenard MP, Basset P, Rio MC