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what is the smoke from cigarettes Happens to Your Body, When You Take a Puff of a Cigarette? Carbon monoxide in cigarette smoke
Description
At suitable time points, colonies were fixed with methanol followed by staining with 0.5% crystal violet in 25% methanol for 10 min

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There is no evidence to prove the same but theoretically defective sperm DNA may be considered as a possible cause for congenital defects in babies born to men who smoke tobacco