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At US$123,000, Hong Kong was 60 per cent costlier than Singapore, the report found
Afterward, the obtained sperm pellets were treated with somatic lysis buffer (SLB: 0.1% SDS, 0.5% Triton-X-100 in DEPC water) on ice for 20 min and then centrifuged at 1000 g for 5 min
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