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Whole-brain c-Fos staining revealed that semaglutide activated 10 brain nuclei, and an overlap between c-Fos signaling and fluorescently labeled semaglutide was observed only in the AP, NTS, and DMX in the brainstem

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Based on the results of the protein-protein interaction analysis, the residues in chain B were categorized into interaction interface residues and non-interaction interface residues