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Description
Cytokine profiling using immunology multiplex assay MCP-1/CCL2, IL-6, and TNF-alpha (TNF) were measured in fasted plasma collected at endpoint using the Milliplex MAP Mouse Metabolic Hormone Magnetic Bead Panel in the Luminex MAGPIX system (EMD Millipore, Billerica, MA)
Libraries were quantified with NuQuant, quality checked using a TapeStation instrument (Agilent Technologies) and subjected to 52 bp paired-end sequencing on the NovaSeq 6000 system (Illumina)
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Baggio and Drucker, 2007
