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Description
The medication being classified as non-essential

For liver IGF-1 measurement, liver tissues were weighed and homogenized in lysis buffer containing PBS, 1% Triton X100 and a protease inhibitor mixture (Roche, cOmplete, EDTA-free Protease Inhibitor Cocktail)

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Experiment 1: Semaglutide Sixteen rats (7 males and 9 females) were initially assigned in a counterbalanced manner to one of four treatment conditions: 0, 0.01, 0.03, or 0.1 mg/kg semaglutide
