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Description
The concentrations of Exe4 and other hepatic markers in plasma were assessed using commercially available assay kits (Exe4: Abcam, AST and ALT: Sigma-Aldrich, Albumin: Fuji Rebis) following the manufacturers protocols, and the absorbance of each sample was measured using a plate reader
Amino Acid Coupling: Each amino acid is coupled to the growing peptide chain via a condensation reaction, typically using carbodiimide or other coupling reagents

It was this process that Eli Lilly chemists used to come up with a commercially viable process for the manufacture of orforglipron
Step 1: Remove the vial from freezer storage and allow it to reach room temperature gradually
