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Plasma concentrations of amylin (active), C-peptide 2, ghrelin, GIP (total), GLP-1 (active), glucagon, insulin, leptin, PP, PYY, resistin, and secretin were measured in duplicate by Eve Technologies using a commercially available Mouse Metabolic Hormone 12-Plex Discovery Assay (MilliporeSigma) according to the manufacturers protocol on a Luminex 200 system (Thermo Fisher Scientific)
it is a major risk factor for global mortality contributing to approximately 5 million deaths annually due to noncommunicable diseases [1]
2017;12:e0181880
