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Wells were washed 3 times with wash solution (0.35 mL per well) before adding 100 L of biotin-labeled polyclonal anti-mouse FGF-21 antibody and incubated on a shaker for 1 hour with captured FGF-21
doi: 10.1006/phrs.2000.0725
It may be worthwhile to investigate thoroughly the role of HMGB1 in causing toxicity to melanocytes to enhance our knowledge of vitiligo and develop newer therapeutic options (Table 2)
By inhibiting mTOR, the energetic switch from aerobic glycolysis to oxphos did not occur, resulting in decreased clonogenic survival (Lu et al., 2015)
