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A suitable quantity of skin tissue (50 mg) was first homogenized and the supernatant after centrifugation (at 4000 rpm for 30 min) was collected

This study also demonstrates that the addition of a carboxylic acid is not essential or crucial to perform epoxidation reactions successfully due to the fact that the unsaturated FAMEs present in the mixture generated by enzymatic hydrolysis act as precursors in the epoxide formation

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Nutrients , 10(3), 349
